| Summary: | 碩士 === 國立成功大學 === 航空太空工程學系碩博士班 === 95 === The area of micro total analysis systems(μTAS), also called “lab on a chip”, or miniaturized analysis systems, is growing rapidly, promise wide applications in biology and chemistry for manipulating samples in suspension to achieve high resolution, fast, and low-cost analysis and synthesis. For these purposes, electric, optical, magnetic, and mechanical forces have been widely used not only as conventional manipulation principles, but also as trapping methods to be integrated in microchip. Dielectrophoresis (DEP), the motion of a particle caused by an applied electric field gradient, can concentrate microorganisms non-destructively. The DEP force as trapping mechanism has the advantage of no risk to the sample. We have developed an alternative method in which a arrangement of insulating trapezoids in a channel of a microchip produce the spatially nonuniform fields for electrodeless dielectrophoretic trapping. Cells are trapped between the trapezoids structures and held against destabilizing flows by dielectrophoretic forces. We have improved the design of electrodeless DEP geometries, correlated particle acted by DEP effects with electrical-field distributions determined through insulating microstructures produce non-uniform electric fields to drive DEP in microsystems. Parameters of structure as being analyzed the effect on the magnitude of DEP force to analysis their influence on the trapping ability. This study is attempt to research on microfluidic trapping by using Dielectrophoresis (DEP) force and to investigate the relation on important parameters of microchip device for our design to comprehend the factors containing the spacing between two structures, the applied voltage and flow velocity.
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