| Summary: | 碩士 === 國立成功大學 === 生命科學系碩博士班 === 95 === Based on the replacement of fossil fuel, finding out the cheap and stable energy is the most important issue for the whole world now. Among all of the alternative energy,
hydrogen can be made of cellulose or other organic material, so has been considered as the major sources of alternative energy in future. Applied both the traditional culture and molecular biologic technique to detect the major hydrogen producing and cellulose degrading bacteria, and observe microbial community structures and dynamic changes in this study. In the fermentative hydrogen-producing bacteria study, four strains of the hydrogen-producing bacteria were isolated from different organic wastes, and microbial community structure in the fermentative reactor was studies and major populations
were ”Clostridium chromoreductans”, Enterobacter asburiae and Citrobacter freundii. In the cellulolytic bacteria study, besides to find the relationship between the cellulolytic bacteria population in the cow feces and the species of Clostridium cluster XIVa, the effects of the microbial community structure with different cellulosic materials enrichment and time of enrichment were observed. In the treatment system of industrial wastewater study, besides the analysis of microbial community structures, the effects of microbial community structures by using different enrichment medium were researched and discussed with DGGE and T-RFLP analysis. Analysis results show that most of the major microbial populations in these samples were belonged to Clostridium cluster I, and played such important roles in fermentative hydrogen-producing reaction of the samples.
|
|---|
