Lipase Immobilized onto Electrospun Nanofibrous Membrane for Hydrolysis of Soybean Oil

• Main Authors: Yu-hsiang Hsu, 徐裕翔
• Other Authors: Wen-teng Wu
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/44437056379756891354

碩士 === 國立成功大學 === 化學工程學系碩博士班 === 95 === Electrospinning, a novel process to make porous nanofiber, was investigated to manufacture nanofibrous membrane as a carrier for Candida rugosa lipase immobilization to hydrolyze soybean oil. In this study, polyacrylonitrile nanofiber with the mean diameter of 200 nm was used to immobilize Candida rugosa lipase by covalent binding. The immobilization efficiency using nanofiber was good. The protein loading of immobilized enzyme on nanofiber was about 60%, and the specific activity of immobilized lipase was 81.3% of the free lipase. In comparison of the kinetic parameter Km of the immobilized lipase with that of the free lipase, the values of Km are similar. It implies that the diffusion resistance of immobilized enzyme is almost the same as that of free enzyme. To compare with other types of immobilized lipase on nanofibrous membrane, the Vmax index of our system was higher than that of others. The effects of temperature, pH and oil to water ratio on the conversion of oil hydrolysis were also illustrated and the optimal operation condition was determined. Under the optimal condition, temperature at 30℃, pH at 7,and oil to water ratio of 2:1 for soybean oil hydrolysis, a conversion of 72% was obtained in 10 minutes.