Fabrication of asymmetric poly(ε-caprolactone) scaffold and cell Co-culture study in-vitro

• Main Authors: Wen-Chen Huang, 黃文成
• Other Authors: Chun-Mei Lu
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/39102579792453822591

碩士 === 國立勤益科技大學 === 化工與材料工程系 === 95 === It is demonstrated that the asymmetric tissue-engineering nerve conduits, with high outflow than inflow rate, will significantly accelerate the bridging of peripheral nerve defects. The improving efficacy of the nerve repaired by the asymmetric structure was attributed to the removal of the blockage of the waste drain from the inner inflamed wound in the early stage. In this study, solvent casting / stratification / particulate leaching techniques were utilized to fabricate controllable asymmetric PCL scaffolds. Three kinds of asymmetrical scaffold (including symmetric PCL-0, asymmetric PCL-037 and PCL-100) were observed by scanning electronic microscopy (SEM). The porosity and pore size of the conduit wall can be controlled by the ratio of the added NaCl particles. The permeability trials indicated that PCL-037 and PCL-0100 scaffolds possessed the high outflow than inflow rate. The results of dynamic mechanical analysis (DMA) showed high elastic modulus, long elongation and high stress in these three scaffolds. The resultant PCL scaffold properties were evaluated by DSC and FT-IR analysis. The in vitro trials indicate good adhesion, proliferation, and low LDH release of L929 and C6 cells on PCL films. Finally, asymmetrical PCL-037 scaffold demonstrated clear directional selectivity to adjust and high controlled permeability of cytokines in the co-culture trial. In the future, the directional transport characteristics should be considered as an important factor to design the optimal nerve conduits.