| Summary: | 碩士 === 國立中興大學 === 獸醫病理學研究所 === 95 === Viral hemorrhagic septicaemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV), and infectious pancreatic necrosis virus (IPNV) are major pathogens listed on OIE notifiable fish diseases among several species of salmonid fish. As the international trade of egg, juvenile, and aquatic animal products between countries become more frequent, it is important to establish more precise and strict inspections under OIE regulation to exclude these pathogens from Taiwan. The aim of this study was to establish several internal positive controls for monitoring sensitivity and specificity during RT-PCR diagnosis. Several plasmids containing the conserved sequence of partial viral genome were constructed and RNAs were synthesized in vitro as internal positive controls for epidemiological surveillance. During July 2006 to March 2007, 160 collected samples including salmons (Salmo salar), rainbow trouts (Oncorhynchus mykiss), sweet fishes (Plecoglossus altivelis), and greater amberjacks (Seriola dumerili) from markets in Taichung area were screened. The results showed IPNV could be detected in rainbow trout and greater amberjacks, but neither IHNV nor VHSV could be found in all tested samples at this moment. Except for molecular biological diagnosis, the immunoserological assay is another alternative method for monitoring infectious fish diseases. However, the variation of antibodies among different fish species may dramatically affect the cross-species recognition of immunoglobulin. Therefore, a rabbit-anti-tilapia immunoglobulin antibody was produced and used to compare the difference of recognition between families of teleost using Western blotting method. These results may provide valuable information for understanding the immune system in different teleost fish.
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