| Summary: | 碩士 === 國立中興大學 === 生物產業機電工程學系所 === 95 === During a microbial culture, the important parameters in bioprocess measurement are pH, temperature, Dissolved Oxygen, oxygen utility rate, carbon dioxide exchange rate, biomass, metabolite concentration and culture medium consumption. However, there is no on-line and fast method to measure biomass, metabolite concentration and culture medium consumption in present stage.
A method is developed and available to measure the oxygen, carbon dioxide concentrations and the flow rate both of in-flow and out-flow of the bioreactor. Then based on these values the model was developed. This model was used to predict the microbial grow rate of biomass. The temperature, mixing speed and ventilated concentration were 22℃, 400 rpm, 0.3 L/min during the cultivations. The inoculums concentration is 7.5％ was followed to measure the cell dry weight.
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