Somatic embryogenesis from cultured developing inflorescence of Hippeastrum in vitro

• Main Authors: Jhih-Jhong Hong, 洪志忠
• Other Authors: 王才義
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/29230724253639798346

碩士 === 國立中興大學 === 園藝學系所 === 95 === To establish an efficient plant regeneration system, this study is to investigate the effects of explants, plant growth regulators and condition on including adventitious shoots, callus, suspension culture and plant regeneration of Hippeastrum ‘Apple Blossom’,‘Blossom Peacock’,‘Lemon Lime’,‘Mrs. Garfield’,‘Red Lion’. The explants of positionⅠand positionⅡ、explant orientation normal and explant in the dark for 4weeks and transfered on the same medium in the light can induce callus and adventitious shoot formation. The explants of ovary and pedicel of Hippeastrum were used for callus and adventitious shoot induction on 1/2MS basal medium containing NAA(0,1,2mg/l) and BA(0,1,2,4mg/l). The results showed that the pedicel was better explants than ovary explants cultured on medium had higher rates of embryogenic calli and adventitious shoots. The best result was found in that ‘Lemon Lime’ pedicel explant formation 6.89 adventitious shoots in medium containing 2mg/l NAA and 4mg/l BA. To induce embryogenic calli and somatic embryogenesis, the immature inflorescence were cultured on the basal media containing 2 mg/l 2,4-D. TDZ 0.0.1-0.1 mg/l could induce the somatic embryo from embryogenic calli. when somatic embryo were culture on the medium without plant growth regulators, somatic embryogenesis, mature and regenerated plantlets could be obtained somatic embryogenesis, mature and regenerated plantlets could be obtained on media containing 30-45g/l sucrose.