Fabrication of tissue engineering PLGA biodegradable Circular Microvessel with nanostructure on the surfaces

• Main Authors: Cheng-Chih Hsueh, 薛丞智
• Other Authors: 王國禎
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/44741689091194055242

碩士 === 國立中興大學 === 機械工程學系所 === 95 === Abstract PLGA (poly(lactic-co-glycolic acid)) is one of the most used biodegradable and biocompatible materials. Nanostructured PLGA even has great application potentials in tissue engineering. In this research, the fabrication techniques for two biomedical PLGA microdevices: (1) PLGA microvessel scaffold with circular microchannels (2) nanostructured PLGA membrane, were investigated and developed. In the fabrication of the PLGA microvessel scaffold with circular microchannels , the thermal reflow technique was adopted to fabricate the semi-cylindrical photoresist master; the PLGA solution was prepared by dissolving PLGA polymer in acetone and then casting the solution onto the semi-cylindrical photoresist master to produce PLGA microstructures; finally two PLGA membranes were bonded together to form microstructures consisting of circular microchannels. A microvessel scaffold suitable for tissue engineering was fabricated using the proposed method, and bovine endothelial cells were cultured into the scaffold by semi-dynamic seeding. The cell stain Calcein-AM was used to overcome the problem of the PLGA scaffolds becoming opaque, which in the past has made it difficult to effectively monitor the progress of cell seeding. In the fabrication of the nanostructured PLGA membrane, an anodic aluminum oxide (AAO) membrane was use as the template; the PLGA solution was then cast on it; the vacuum air-extraction process was then applied to transfer the nano porous pattern from the AAO membrane to the PLGA membrane and form nanostures on it. The cell culture experiments of the bovine endothelial cells demonstrated that the nanostructured PLGA membrane can double the cell growing rate. Compared to the conventional chemical-etching process, the physical fabrication method proposed in this research not only is simpler but also does not alter the characteristics of the PLGA. The nanostructure of the PLGA membrane can be well controlled by the AAO temperate. The two biomedical PLGA microdevices developed in this research are novel both in the fabrication techniques and the structures. They have very feasible applications in tissue engineering.