Production of Infectious Bursal Disease Virus Vaccine byBamboo Mosaic Virus-Based Vector

• Main Authors: Ssu-Shin Yu, 余思賢
• Other Authors: 徐堯煇
• Format: Others
• Language: en_US
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/24873208244923794939

碩士 === 中興大學 === 生物科技學研究所 === 95 === Infectious bursal disease virus, IBDV, is the major cause for infectious bursal disease of chickens. Two strategies are applied in our experiments, fusing either short peptides or the intact P-domain of VP2 with the N-terminal of BaMV coat protein as antigens. The stability and yield of chimeric virus particles loaded with antigens are examined by inoculating these chimeric BaMV clones into plants. In order to facilitate the assembly of chimeric BaMV with the intact P-domain, we inserted a FMDV 2A sequence to bridge the BaMV coat protein from the vvIBDV P-domain sequence. The active auto-cleavage of the 2A sequence renders the assembled chimeric virus particles with only a small amount of fusion protein. After careful evaluation of the recombinant proteins by using western blot analyses, we selected three BaMV chimeric clones with peptide fusions and inoculated plants, then purified the virus particles as antigens for animal tests. In our preliminary results, two of the three chimeric BaMV vaccines showed moderate protection ability. The yields of chimeric BaMV particles containing the intact P-domain in plants were low; most of the P-domain was cleaved. Further adjustment of 2A cleavage activity is needed.