| Summary: | 碩士 === 中興大學 === 生物科技學研究所 === 95 === Infectious bursal disease virus, IBDV, is the major cause for infectious bursal disease
of chickens. Two strategies are applied in our experiments, fusing either short
peptides or the intact P-domain of VP2 with the N-terminal of BaMV coat protein as
antigens. The stability and yield of chimeric virus particles loaded with antigens are
examined by inoculating these chimeric BaMV clones into plants. In order to
facilitate the assembly of chimeric BaMV with the intact P-domain, we inserted a
FMDV 2A sequence to bridge the BaMV coat protein from the vvIBDV P-domain
sequence. The active auto-cleavage of the 2A sequence renders the assembled
chimeric virus particles with only a small amount of fusion protein. After careful
evaluation of the recombinant proteins by using western blot analyses, we selected
three BaMV chimeric clones with peptide fusions and inoculated plants, then purified
the virus particles as antigens for animal tests. In our preliminary results, two of the
three chimeric BaMV vaccines showed moderate protection ability. The yields of
chimeric BaMV particles containing the intact P-domain in plants were low; most of
the P-domain was cleaved. Further adjustment of 2A cleavage activity is needed.
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