The biological and molecular study of Saussurea involucrata inhibits human breast cancer cells proliferation

• Main Authors: Hsiu-Shan Lin, 林秀姍
• Other Authors: Jung-Yie Kao
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/96602577257467205286

碩士 === 中興大學 === 生命科學院碩士在職專班 === 95 === Saussurea involucrate is a well-known Chinese herbal which holds many therapeutic effects. Folk applications include easing rheumatoid arthritis, free-radical clearance, anti-tumor and anti-hypertension. Saussurea involucrate, containing bunch of alkaloids and flavonoids, was also used as an anti-inflammatory drug in Chinese medical application. In this study, we focus on the anti-tumor effect of Saussurea involucrate. We compare the growth inhibition of aggressive breast cancer cell line MCF-7, MDA-MB-453 and normal breast epithelial cell HBL-100. Results showed that both MCF-7 and MDA-MB-453 are more sensitive than HBL-100 to the extract of Saussurea involucrate treatments, for IC50 being 100μg/ml. We also examine the cell cycle alternation by flow cytometry. We found that the extract of Saussurea involucrate could arrest MCF-7 cell cycle on the G0/G1 phase, therefore block its proliferation. Furthermore, we used western blot to assay the concentration discrepancy of G0/G1 phase-associate proteins P21, P27, P53, cyclin D1, CDK4, p-Erk 1, p-Erk 2 and p-JNK. We found that P21, P27, P53 have significant elevation during 100μM Saussurea involucrate treatment, while CDK4 and p-JNK remain constant and cyclin D1, p-Erk 1, p-Erk 2 are decreased during the treatment. In conclusion, the active ingredients of Saussurea involucrate could block breast cancer cell growth by stopping cell cycle progression.