| Summary: | 博士 === 中興大學 === 生命科學系所 === 95 === Natriuretic peptides (NPs) are natural antagonists to the endocrine
system, and they serve important roles in regulating blood volume and
pressure, muscle hypertrophy, pulmonary hypertension, fat
metabolism, and long bone growth. Although the physiologic actions of
NPs in humans have been elucidated, the influence of NPs upon the
mechanism of gene regulation in the renal system is not fully
understood. This study aims to investigate the biological effect of NPs
on gene expression profiling in LLC-PK1 renal tubular epithelial cells,
and their influence on the mechanism of gene regulation in the renal
system. Using DNA microarray analysis, the gene expression profile of
atrial natriuretic peptide (ANP) treated LLC-PK1 cells provided 807
differentially expressed genes, containing 483 up-regulated genes and
324 down-regulated genes. In another model, the gene expression
profile of C-type natiuretic peptide (CNP) treated LLC-PK1 cells
provided 613 differentially expressed genes, containing 312
up-regulated genes and 301 down-regulated genes. The gene ontology
of all differentially expressed genes could be classified according to
cellular component, molecular function and biological process, which
are useful information for functional gene categorization. Interestingly,
four ANP-responsive genes (ATP1B1, H3F3A, ITGB1 and RHO) and
nine CNP-responsive genes (ANKRD17, CD1A, CKS2, HSPCA,
ITGB1, TMEPA1, TRA2A, ZF and ZNF265) were up-regulated genes
while only two CNP-responsive genes (NRAS and NRN1) were
down-regulated genes. In both ANP and CNP treated models, all of the
responsive genes may contribute to alleviation of progressive renal
hypertrophy. Based on the observed pathophysiologic correlations
under these models, gene expression profiling may become useful
differential markers for future diagnostic and therapeutic applications
in renal failure or other related diseases.
|
|---|
