| Summary: | 碩士 === 國立中興大學 === 化學系所 === 95 === We introduce here the application of barrel plating technology for developing disposable-type electrodes; the barrel plated electrode is then further used for electroanalysis and demonstrating electrochromism. The experimental section mainly deals with activation of the electrode with two step process; at first the barrel-plated nickel electrode(Ni-BPE) is thoroughly washed with a surfactant and follows an electrochemical activation. Here we use Triton X-100 as a surfactant for washing and electrochemical activation is carried out by using 0.1 M NaoH solution. Depending up on the nature of surfactant and pH of the NaOH solution, degree of activation of Ni-BPE is varied.
The activated Ni-BPE is used for the application of IMAC (immobilized metal ion affinity chromatography) coupled with electrochromic effect. The separation and purification of protein is a hot subject in the fast growing research filed of biotechnology, Immobilized metal ion affinity chromatography is a separation technique that is based on coordinate covalent binding between proteins and metal ions. We believe that simply by switching the nickel ion valance between Ni+2/Ni+3, Ni-BPE can be used in immobilized metal ion affinity chromatography. Proteins with the Hex-His residues can be accumulated to the Ni-BPE through the formation of nickel-histidine bond.
A disposable Ni-BPE is used for sensitive determination of Cr(III). In the alkaline solution, the Ni-BPE with the high valence Ni3+ states shows good catalytic ability to determine Cr(III). Flow injection analysis was then performed by using Ni-BPE. Under the optimal condition Ni-BPE shows a linear calibration range 5 uM-1 mM with current sensitivity and regression coefficient of 0.00867 uA/uM and 0.998, respectively. In addition to that ten repetitive injections of 300 uM Cr(III) results relative standard deviations of less than 5%, indicate good reproducibility of the present system for determination of Cr(III).
Since the fabrication cost of BPE is being cheap and disposable in nature makes way for easy mass production of electrode. Based on the above facts, In future Ni-BPE can further used for a practical application in electroanalytical chemistry.
|
|---|
