The Analysis of Glucose in Urine by HPLC with Evaporative Light-Scattering Detector (ELSD)

• Main Authors: Lu-Mo Huang, 黃綠模
• Other Authors: Shihn-Sheng Wu
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/46554807156693064565

碩士 === 高雄醫學大學 === 藥學研究所碩士班 === 95 === 英文摘要 An analytical method for determination of glucose in urine has been developed. Glucose and maltose, the internal standard (I.S.), were analyzed by high performance liquid chromatography (HPLC) with evaporative light-scattering detector (ELSD). Separation was performed on a Prevail Carbohydrate ES column (5 μm, 250 mm × 4.6 mm) with acetonitrile : H2O at the rate of 80 : 20% (v/v) with flow rate of 1.2 mL/min. ELSD conditions were optimized at nebulizer-gas flow rate of 1.0 L/min and drift tube temperature of 100 ℃. For glucose in urine the inter-assay precision was 3.81% at 60 μg/mL, 2.76% at 120 μg/mL, 1.98% at 320 μg/mL. The relative errors (R.E.) and relative standard deviations (R.S.D.) were all ≦ 2.16 % and 3.81 %, respectively. The limit of quantitation (LOQ) was determined to 40 μg/mL. Linearity was found in the range of 40~480 μg/mL with a correlation coefficient of 0.9992 ± 0.0001. The mean value of slope and intercept were 0.0025 ± 0.0001 and 0.0093 ± 0.0037, respectively. The developed analytical procedure was successfully applied to determine glucose content in urine samples from 31 communities in Sanmin District of Kaohsiung city.