| Summary: | 碩士 === 高雄醫學大學 === 醫藥暨應用化學研究所碩士班 === 95 === Chondroitin Sulfate (CS), with a good compatibility and degradable chemical structure, can be used in connective tissues and cartilage. polyethylene glycols (PEG) is non-toxic and well biocompatible, which is useful in medical treatments. In drug-cover design, the copolymer hydrogel that is the compound of the above two chemicals entraps the bioactive moleculars in the gel by physical means, and the network-like structural pores of the hydrogel are used for drug delivery. Therefore, the design of this experiment intends to modify PEG and CS by introducing acryloyl chloride (DA) and methacrylic anhydride (MA) as agents to make the above two copolymers be modified with double bonds, and photopolymerization is applied to creat hydrogels, where the molecular masses of PEG are 1000, 3400, 8000 and 20000 g/mol. Then compression tester is used on the hydrogel created to observe the cross-linking density; X-ray diffraction (XRD) is used to investigate the crystallization; Differential scanning calorimetry (DSC) is used to study the melting point of the hydrogels; and Scanning electron microscope (SEM) is used to examine the density of pore formation and microstructure. For these hydrogels, in vitro simulation is conducted to simulate the environment in
a human colon to investigate the use of hydrogel-encapsulated bovine serum albumin (BSA) in control drug release. From the experiment result, it is clear that pore sizes, the degree of swelling, the degree of crystallization and the drug released from the hydrogels increased with the increased of molecular weights of PEG, and yet there is no significant change in the physical characteristics of the hydrogel created with PEG different molecular weights and CS-MA at the same identical molar ratio.
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