Study on immobilized glucoamylase on bacterial cellulose

• Main Authors: Ying-ke Lia, 賴盈課
• Other Authors: Sheng-chi Wu
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/30273159906412678169

碩士 === 輔英科技大學 === 生物技術系碩士班 === 95 === Abstract Glucoamylase was immobilized onto bacterial cellulose bead activated with five different methods. The immobilized glucoamylase presented better performance than free enzyme when submitted to heat treatment, showing a 10 ◦C decrease on the optimal assay temperature. The optimum condition such as reaction pH is 4.5and free enzyme is pH 5. The immobilization enhanced the enzyme stability against changes of pH value and temperature. The activity of immobilized enzyme was also improved 15% by optimal 1,4-butanediyl diglycidyl ether concentaration. The Km value of free enzyme for starch (3.025 g/L) was higher than that of the immobilized enzyme (2.667 g/L)﹒ Finally﹐the enzyme was also immobilized on bacterial cellulose by periodate oxidation mothod and the optimal conditions was searched. In the optimum temperature, the method performed equally with that of the preceding method. Moreover﹐the activity of immobilized enzyme retained more than 95% of relative activity when the immobilization was performed during pH3-5.