Characterization and Functional analysis of mutated plant PR5 genes obtained from DNA shuffling method

• Main Authors: Chiung-Fang Hsu, 許瓊芳
• Other Authors: Ruey-Chih Su
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/82666986163632254802

碩士 === 輔仁大學 === 生命科學系碩士班 === 95 === Plant pathogenesis-related group 5(PR-5) proteins include thaumatin, osmotin, and their related proteins. The PR-5 proteins are known to accumulate in plant in response to pathogen invasion or environmental stress. Many PR-5 proteins have been shown to possess antifungal activity. Although the specific function of PR-5 proteins in plants is still largely unknown, they are reported to cause the inhibition of hyphal growth and reduction of fungal spore germination . In order to study the antifungal activity of PR-5 proteins, DNA shuffling technique were applied in this study to randomly recombind two homologous PR5 genes, namely, pA13 (Solanum commersonii) and PR5 (Oryza sativa) genes. We have obtained five (1301, 13502, 501, 51306, 51310) shuffled PR-5 proteins expressed in Escherichia coli. The phylogenic tree analysis illustrated that among the obtained shuffled proteins,all but except one 501, are close to pA13 (osmotin-like) protein. The shuffled proteins showed variable growth inhibition to Saccharomyces cerevisiae, and the protein 51306 had the best growth inhibition in pH7.5. However all of the proteins have no inhibitory effect to fungal pathogen Rhizoctonia solani in this study.