| Summary: | 碩士 === 大葉大學 === 生物產業科技學系 === 95 === Antrodia cinnamomea is a indigenous fungus in Taiwan, it was also well known as one of the most expensive medicinal mushrooms effective for anticancer, antitumor and liver protection. For the present time, it is well known that polysaccharides and triterpenes are major effective compositions in this medicinal fungus. The objectives of this research were to study different effects of Chinese medicinal herbs and essence on solid culture of A.cinnamomea. The mycelium diameter and growth were investigated at different culture intervals. At the end of culture, the mycelia were freeze-dried and its triterpenes and polysaccharides were analyzed by High Performance Liquid Chromatography. The bio-diversion of the compositions in Chinese medicinal herbs and essence into bioactive substances by A. cinnamomea were expected. Research on polysaccharides showed that medium supplemented with Perilla(10g/L) and Safrole(0.5％) resulted in higher polysaccharide content as 20.29 mg/ml and 13.86mg/ml, respectively, with its bio-diversion rates of 2.22 and 1.80 respectively. As triterpenoids were analysed, addition of Magnolia officinalis (5g/L) and Ganoderma lucidum (20g/L) to the basic medium had bio-diversion rates of 4.05 and 4.93, respectively. Data from HPLC, revealed that addition of Ganoderma lucidum (20g/L) resulted in the highest triterpenes content of 37.61mg/g in mycelia in 30-days culture. Basil, Magnolia officinalis and Perilla also increased triterpenes content in cultured mycelia efficiently. Therefore, this experiment provided some substitutes for woods of Cinnamomum kanehirai to culture A. cinnamomea with high bio-activity products.
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