| Summary: | 碩士 === 大葉大學 === 生物產業科技學系 === 95 === Hyaluronic acid is a linear polysaccharide composed of repeating subunits ofβ-1,4-linked disaccharide of glucuronic acid β-1,3- N-acetylglucosamine. It has been existed in extracellular matrix in animal tissue. There are close relations in capsule and hyaluronidas .The studies have demonstrated the role played by reactive oxygen-dericed species in depolymerization of hyaluronic acid (HA) , that antioxidants would protect HA from oxidative degradation. The effect of various cultures on temperature, pH, agitation rate and added various antioxidants concentration of hyaluronic acid production by Streptococcus zooepidemicus was studies. And the effects of extracellur hyaluronic acid and capsule hyaluronic acid were investigated. Morphological details of the sample were evaluated using and image analyzer. The result exhibited that extracellur hyaluronic acid concentration of 37℃(1.45 g/L)、pH 7.5 (1.74 g/L)、150 rpm(1.57 g/L)、50 mg/kg of gallic acid (1.58 g/L)、250 mg/kg of ascorbic acid (1.54 g/L)、250 mg/kg of tocopherol (1.49 g/L) had the maximum yield. The capsule hyaluronic acid had the maximum yield in 37℃(0.08 g/L)、pH 7.5(0.08 g/L)，150 rpm(0.09 g/L). On the other hand, the capsule were significant increased in 6h by culture time.
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