| Summary: | 碩士 === 朝陽科技大學 === 生物技術研究所 === 95 === The aim of this study is to establish a stable metabolic characteristic of non-insulin dependent diabetes mellitus (NIDDM) animal model. Two days old rat pups of Sprague-Dawley (SD) were used in this experiment. The pups were administered with 40, 70, 100 and 130 mg/kg streptozotocin (STZ), by intraperitoneal method, respectively. Different dosage of STZ, solution were diluted by dissolving in 0.1M citrate buffer (pH=4.5). The rats received citrate buffer only in control group. The induced diabetes rats were divided into two test groups as rat growing at 5 week old. One group was fed standard diet until the end of the test. The other group was given high-fat diet (TD96132, Harlan Teklad) for two weeks, then fed the standard diet again until the end of the test. The rats administered STZ 70, 100 and 130 mg/kg and standard diet show significantly increasing in concentration of plasma glucose compared to control group(p<0.05). The rats exhibited high plasma glucose beyond 400 mg/dL, when they treated with high-fat diet combined STZ 100 and 130 mg/kg, respectively. HbA1c concentration of treated 10 week old rats as administered with 100 and 130 mg/kg combined standard or high-fat diet significantly increased as compared to the blank control (p<0.05). Immunohistochemistry assays showed the morphology of pancreatic βcell in the pancreatic islet were not absent. The concentration of plasma insulin of rat as administered with STZ 40 and 70 mg/kg conbined with standard or high fat diet, respectively were significantly increased as compared to control group. The experiment also used SDS polyacrylamide gel electrophoresis (SDS-PAGE) and western blotting method to detect the concentration of GLUT4 protein and total protein content of muscle and adipose tissue. The concentration of GLUT4 protein decreased in skeletal muscle of treated rat under 100 or 130 mg/kg STZ, but not in adipose tissue. There were no significantly different between high fat and standard diet group rat.
On the other test, two days old SD pups rats were induced by 130mg/kg STZ with intraperitoneal injection method. After 5 week, the diabete induced rats were fed high-fat diet. After 7 week, those rats were divided into two groups. One was treated with 0.134 mg/kg/day rosiglitazone by gavage for 3 week. The other group was treated with saline only.The blood glucose level, HbA1c and triglycerides were significantly decreased as compared to 130 mg/kg STZ induced rats group. Other wise, the level of choloesterol in rosiglitazone treated group after 1 week had no significantly different as compared to 130 mg/kg STZ induced rats. A NIDDM animal model have been successfully developed in this study.
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