| Summary: | 碩士 === 中原大學 === 化學工程研究所 === 95 === Abstract
In this study, the effect of MG63 osteoblast cells cultured on a Polycaprolactone (PCL) membranes surfaces with different pore sizes was investigated. PCL membranes were prepared by adding hydrophilic PEG300 into polymer solution, and then used wet induced phase separation process to create the asymmetric membrane with microporous surface and macroporous bottom structure. Microporous structure can resist the faster proliferation epidermal cells and the macroporous bottom structure can provide room for osteoblasts proliferation and further form the bone structure. By this study, we may find an optimized membrane pore size for bone cell regeneration. Base on this research, this polymer membrane can be used on the application of guided tissue regeneration (GTR) and guided bone regeneration (GBR).
Adding 5-20 wt% PEG300 additive into the polymer solution and then prepared the PCL membrane with microporous surface and uniform pore size between 50 and 100μm. However, PEG content cannot generate obvious difference in the pore size while membrane thickness is the major factor for macroporous pore size. If film thickness is 53μm, 120μm, 239μm and 535μm, after “ “ simplePCI ” software analysis of membrane porous structure, we found that the pore numbers decreased while the membrane thickness increased, which means the final pore size are 23.3±5.6μm,74.7±14.2μm, 158±43.7μm and 203±45.9μm respectively, and pore numbers are 1342、158、29 and 15 pore/mm2 respectively.
SEM, MTT assay and ALP activity assay were utilized to investigate porous PCL membrane structure with 1,3,7 and 19 days osteoblasts cell culture. The result showed that osteoblasts cell with spread characteristics already attached onto the porous membrane surface, but after 1 day culture, we found it took 3 days to observe the same phenomena on the microporous surface (pore size 0.14±0.04μm). Thus we can conclude that microporous surface is not suitable for the spreading of osteoblasts. By MTT assay result, PCL with 74.7±14.2μm pore size has fastest proliferation rate. According to ALP activity assay result, no obvious ALP level difference was found by changing membrane thickness and pore size. Finally, we also found that all PCL membrane structures can induce the differentiation of Osteoblasts and the most optimum membrane structure for Osteoblast growth is 120μm membrane thickness and 74.7±14.2μm pore size.
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