Development of Light-addressable Multi-electrode Arrays Chip and Multi-channel Recording System

• Main Authors: Pei-Kai Hung, 洪培凱
• Other Authors: none
• Format: Others
• Language: en_US
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/12733994824579024952

碩士 === 中臺科技大學 === 醫學工程暨材料研究所 === 95 === In this study, a light addressable microelectrode array (LA-MEA) chip and multichannel recording system has been developed to measure electrophysiological signals of neural cells. A sputtered indium tin oxide (ITO) film on a glass is to substitute for metal electrodes and leads in this LA-MEA chip. Hydrogenated amorphous silicon (a-Si:H) is then deposited above the ITO layer and serves as a photoconductor layer. Vertical cavity surface emitting laser (VCSEL) is used as light source and is structured in 8 by 8 arrays with a distance of 200 μm to each other. Each ITO electrode on the chip is addressed by the corresponding VCSEL diode. Meanwhile, the material property of micro-electrode embedded on LA-MEA chip is verified to investigate the optimal fabrication parameters. The result shows the photoconductor micro-electrode of LA-MEA system possesses higher impedance but neural signals are accessible in this system. The specific neural precursor cell line, PC-12, is used in our experiments to measure the electrophysiological signals.