Effect of biflavanoids on three cancer cells

• Main Authors: Ya-Ting Liu, 劉雅婷
• Other Authors: Rong-Fong Lin
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/89390377012415623834

碩士 === 中臺科技大學 === 醫學生物科技研究所 === 95 === Flavanoids have been reported to inhibit proliferation or result in apoptosis of cancer cells. However, effects of biflavanoids on cancer cells have not yet been described. In this study, three kinds of biflavanoids hinokiflavone, robustaflavone, and amentoflavone were used to treat human cancer cell lines TE671 (rhabdomyosarcoma), DBTRG (brain glioblastoma), and A549 (lung adenocarcinoma). The IC50 of hinokiflavone on TE671 and DBTRG were 5.8 μM and 40.3 μM, respectively, but that on A549 was more than 74.4 μM. On the other hand, the IC50 of robustaflavone and amentoflavone on TE671, DBTRG, and A549 were all more than 74.4 μM. The effect of hinokiflavone on growth inhibition of cancer cells was most significant. Therefore, the studies of the mechanism of action on inhibiting cancer cells by hinokiflavone was conducted. RT-PCR analysis revealed that the expression of brca2 mRNA was inhibited in the three cell lines. No DNA fragmentation was found in the three cell lines. The protein levels of p53 and caspase 9 increased in hinokiflavone-treated TE671, DBTRG and A549. The active form of caspase 3 was detected. Therefore, the inhibition of hinokiflavone aganist cancer cells possibly is through apoptosis.