Effect of biflavanoids on colon cancer cells

• Main Authors: Yuan-chen Chao, 趙元禎
• Other Authors: Rong-fong Lin
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/01900141453233356135

碩士 === 中臺科技大學 === 醫學生物科技研究所 === 95 === Biflavanoids hinokiflavone, robustaflavone, and amentoflavone were isolated and purified from Rhus succedanea L. Three colon cancer cells HT-29, SW-620, and a primary cultured cell were treated with the three biflavanoids. The results indicated that hinokiflavone revealed significant growth inhibition on colon cancers. The inhibition concentrations were 37 nM on HT-29 and SW-620, and 18 nM on primary cultured cell. However, no significant effects of robustaflavone and amentoflavone were present. Survival rate of colon cancer cells with hinokiflavone treatment decreased through MTS assay. Experiments with RT-PCR indicated that mRNA levels of bcl-2, p53, brca2, and cox-2 were lowered after treatment with hinokiflavone. Protein levels of Caspases 6, 8, and 9 elevated after one day treatment by western analysis. The above results showed that hinokiflavone might result in apoptosis of colon cancer cells.