Comparison of biocompatibility of pulpotomy materials

• Main Authors: Chao-Hsin, 劉兆欣
• Other Authors: Tsui-Hsien Huang
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/15762461527208228445

碩士 === 中山醫學大學 === 口腔材料科學研究所 === 95 === Objectives: The purpose of present study was to compare the biologic effects of Zinc oxide eugenol cement (ZOE) or Calcium hydroxide（CH） mix with Formocresol（FC） or Ferric sulfat（FS） or Glutaraldehyde（GA） and Mineral Trioxide Aggregate（MTA） Methods: Total seven materials were prepared according to clinic application and immersed in MEM medium for 24 hours. The prepared materials were treated on NCTC clone 929 (L929) and analyzed by MTT assay to detect the cell survival rate, by DNA fragmentation assay to analysis its gene toxicity and by Western blot assay to detect the signal transduction pathway of treated cells. Results were compared using one way analysis of variance (ANOVA). Differences in treatment means were analyzed using Student-Newman-Keul’s test and were considered statistical significance at P<0.05. Results: All experiment groups showed cytotoxicity when treated with L929 cells when compared with control group. (P＜0.05) The cytotoxicity were increased as concentrations and treated time increased. (P＜0.05) There do not show any DNA ladder in all experimental grouops. The L929 p53 expression showed time dependent increased in experimental groups. (P＜0.05) Conclusion: From in vitro study showed that MTA or CH mixed with GA which existed lower toxicity. They can be applied as pulpotomy material in clinic.