Study of Relationships Between Kidney Allograft Fibrosis and Serum Proteinases

• Main Authors: Chiu-Chu, 林秋菊
• Other Authors: Yih-Shou Hsieh
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/56236136740231929510

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 95 === The two most common causes of kidney allograft failure are allograft dysfunction and chronic allograft nephropathy (CAN). One way to characterized CAN is by the presence of renal fibrosis. Although matrix metalloproteinases (MMPs) and plasminogen activator (PA)/ plasmin system plays a key role in the progression of renal fibrosis; the exact relationship between kidney allograft fibrosis with MMPs, u-PA and PAI-1 still remains unclear. In trying to correlate kidney allograft fibrosis with the above parameters, fifty kidney transplant recipients were enlisted in our study. Samples were collected from the recipients’ kidney allograft tissue obtained from percuataneous renal biopsy, serum and urine. The chronic allograft damage index (CADI) score was adopted as a measurement of kidney allograft fibrosis. MMP-2 and MMP-9 activity was measured by a method based on gelatin zymography, while u-PA and PAI-1 levels were detected with ELISA. Our results demonstrate that after a period of time, kidney allograft fibrosis severity increased with increasing transplantation duration (p<0.001), in addition, there is a linear dependence between CADI score and serum PAI-1 (p=0.003). The current study suggests clinicians can use correlated serum PAI-1 data as a mean to diagnose CAN and determine the severity of kidney allograft fibrosis. By developing an early detection method, the survival rate for CAN patients may be increased significantly.