| Summary: | 碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 95 === The aim of the work was to investigate the antioxidant and hepatoprotective effect of water extract of napiergrass (WEN). In the first part, the antioxidant and the effect of WEN, rutin and p-coumaric acid on biomolecules oxidation were explored in vitro. The results showed that WEN, rutin and p-coumaric acid protected liposome, deoxyribose and low-density lipoprotein (LDL) from oxidation, and scavenged free radicals including hydrophilic ABTS+．, hydrophobic DPPH and NO produced from SNP. WEN, rutin and p-coumaric acid showed reducing ability in dose-dependent manners, respectively. Besides WEN, rutin and p-coumaric acid were not exhibited ferrous chelating activity. According to data obtained, WEN, rutin and p-coumaric acid show antioxidant and protective from biomolecules oxidation. Second, WEN was evaluated for hepatoprotective activity in rats by inducing liver damage by CCl4. The results showed CCl4-treated group with increasing relative hepatic weight, increasing serum GOT, GPT, and increasing hepatic GSH and MDA levels. Furthermore, the decrease of antioxidant enzyme activities were not significant (p<0.05) after CCl4 treatment. On the other hand, WEN fed for 13 days alleviated the increase of GSH and MDA, respectively, in rats treated with CCl4. But serum biochemical parameters and antioxidant enzyme activities were not obviously decreased by feeding WEN for 13 days in CCl4 treat rats. However, when the rats were fed WEN for 28 days, the decreasing of serum GOT and hepatic SOD activity were observed. Overall, WEN had in vitro protective action on biomolecules oxidative damage. WEN could increase GSH content and decrease liver peroxidation against CCl4 damage. However, the protection of WEN to decrease CCl4 induced hepatotoxicity is probably not involved the regulation of enzymatic antioxidants system.
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