Chalcone arrests cell cycle progression and induces apoptosis through induction of mitochondrial pathway and inhibition of nuclear factor κB signaling in human bladder cancer cells
碩士 === 嘉南藥理科技大學 === 生物科技研究所 === 95 === Chalcones are cancer preventive food components found in a human diet rich in fruits and vegetables. In this study, we first report the chemopreventive effect of chalcone (1,3-Diphenyl-2-propenone) in two human bladder cancer cell lines, T24 and HT-1376. The re...
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ndltd-TW-095CNUP51110182015-10-13T15:42:15Z http://ndltd.ncl.edu.tw/handle/89281330934344048175 Chalcone arrests cell cycle progression and induces apoptosis through induction of mitochondrial pathway and inhibition of nuclear factor κB signaling in human bladder cancer cells 苯基苯乙烯酮對膀胱癌細胞之活性與其作用機轉之探討 Kun-Hung Shen 沈坤宏 碩士 嘉南藥理科技大學 生物科技研究所 95 Chalcones are cancer preventive food components found in a human diet rich in fruits and vegetables. In this study, we first report the chemopreventive effect of chalcone (1,3-Diphenyl-2-propenone) in two human bladder cancer cell lines, T24 and HT-1376. The results show that chalcone inhibits the proliferation of T24 and HT-1376 cells by inducing apoptosis and blocking cell cycle progression in the G2/M phase. Western blot assay showed that chalcone significantly increases the expression of p21 and p27 proteins, and decreases the levels of cyclin B1, cyclin A and Cdc2, thereby contributing to cell cycle arrest. In addition, chalcone increased the expression of Bax and Bak, but decreased the levels of Bcl-2 and Bcl-XL and subsequently triggered mitochondrial apoptotic pathway (release of cytochrome c and activation of caspase-9 and caspase-3). Our study suggests that the induction of mitochondrial pathway and inhibition of the NF-κB survival system may play important roles in the antiproliferative activity of chalcone in T24 and HT-1376 cells. Jiunn-k ae Chang Po-lin kuo 張竣凱 郭柏麟 2007 學位論文 ; thesis 59 zh-TW |
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碩士 === 嘉南藥理科技大學 === 生物科技研究所 === 95 === Chalcones are cancer preventive food components found in a human diet rich in fruits and vegetables. In this study, we first report the chemopreventive effect of chalcone (1,3-Diphenyl-2-propenone) in two human bladder cancer cell lines, T24 and HT-1376. The results show that chalcone inhibits the proliferation of T24 and HT-1376 cells by inducing apoptosis and blocking cell cycle progression in the G2/M phase. Western blot assay showed that chalcone significantly increases the expression of p21 and p27 proteins, and decreases the levels of cyclin B1, cyclin A and Cdc2, thereby contributing to cell cycle arrest. In addition, chalcone increased the expression of Bax and Bak, but decreased the levels of Bcl-2 and Bcl-XL and subsequently triggered mitochondrial apoptotic pathway (release of cytochrome c and activation of caspase-9 and caspase-3). Our study suggests that the induction of mitochondrial pathway and inhibition of the NF-κB survival system may play important roles in the antiproliferative activity of chalcone in T24 and HT-1376 cells.
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author2 |
Jiunn-k ae Chang |
author_facet |
Jiunn-k ae Chang Kun-Hung Shen 沈坤宏 |
author |
Kun-Hung Shen 沈坤宏 |
spellingShingle |
Kun-Hung Shen 沈坤宏 Chalcone arrests cell cycle progression and induces apoptosis through induction of mitochondrial pathway and inhibition of nuclear factor κB signaling in human bladder cancer cells |
author_sort |
Kun-Hung Shen |
title |
Chalcone arrests cell cycle progression and induces apoptosis through induction of mitochondrial pathway and inhibition of nuclear factor κB signaling in human bladder cancer cells |
title_short |
Chalcone arrests cell cycle progression and induces apoptosis through induction of mitochondrial pathway and inhibition of nuclear factor κB signaling in human bladder cancer cells |
title_full |
Chalcone arrests cell cycle progression and induces apoptosis through induction of mitochondrial pathway and inhibition of nuclear factor κB signaling in human bladder cancer cells |
title_fullStr |
Chalcone arrests cell cycle progression and induces apoptosis through induction of mitochondrial pathway and inhibition of nuclear factor κB signaling in human bladder cancer cells |
title_full_unstemmed |
Chalcone arrests cell cycle progression and induces apoptosis through induction of mitochondrial pathway and inhibition of nuclear factor κB signaling in human bladder cancer cells |
title_sort |
chalcone arrests cell cycle progression and induces apoptosis through induction of mitochondrial pathway and inhibition of nuclear factor κb signaling in human bladder cancer cells |
publishDate |
2007 |
url |
http://ndltd.ncl.edu.tw/handle/89281330934344048175 |
work_keys_str_mv |
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