| Summary: | 碩士 === 嘉南藥理科技大學 === 生物科技系暨研究所 === 95 === The role of trace elements in physiologic functions including: stabilizer, elements of structure, essential elements for hormonal function, and cofactors in enzymes. If these trace elements are out of balance, cells will damage and cause diseases. The unregulated growth that characterizes cancer is caused by damage to DNA, resulting in mutations to genes that encode for proteins controlling cell division. These mutations can be caused by chemicals, radiation or oncogenic virus that causes cancer, which are called carcinogens. The recent 10 years to discover the trace elements are relate to cancer such as iodine and thyrophyma.
In Taiwan, cancer is the top 1 death reason and the incidence of bladder cancer increase gradually. In this study, detection of trace elements namely copper, zinc, selenium, arsenic, and lead in urine of bladder cancer patients and healthy adults using graphite furnace atomic absorption spectrophotometer (GFAAS). Findings the concentration of zinc and selenium in urine for bladder cancer patients were higher than for healthy adults (P<0.05). Because the ratio of zinc/selenium (138.96<434.92) at superficial bladder cancer is lower than invasive bladder cancer that urinary zinc/selenium ratio will contribute one method to bladder cancer early detection. The urinary zinc concentrations in invasive bladder cancer patients are higher than superficial bladder cancer patients and healthy adults, but urinary selenium concentration in superficial bladder cancer patients are higher than invasive bladder cancer patients and healthy adults. This data shows that zinc and selenium are relationship with bladder cancer cells growth.
In vitro, supply zinc gluconate(>1000 ppb) and sodium selenate(>10 ppb) to culture medium were influence cells survival rate (<50％) by MTT assay. The results of cell migration assay show that supply zinc gluconate(60 ppb) and sodium selenate(3 ppb) to culture medium were inhibit cell migration within 24 hours. And the MMP-9 levels were decrease with time increase by western blotting. Supply zinc gluconate or sodium selenate to TSGH 8301 cells were increase cell apoptosis and detected by flow cytometry. Trace elements therapy use in superficial bladder cancer patients better than invasive bladder cancer patients, yet the feasibility trace elements therapy need to test and verify by clinical study.
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