Pharmacognostical studies on Centipeda minima in Taiwan

• Main Authors: Huai-Ching Tseng, 曾懷慶
• Other Authors: 邱泰惠
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/45962799566166377250

碩士 === 中國醫藥大學 === 藥學系碩士班 === 95 === The genus Centipeda (Compositae) is distributed in low altitude area of tropics and subtropics and has about 4 to 6 species in the world, but only has one species in Taiwan. This species is Centipeda minima. According to the reports, it can relieve symptoms in the nose and lungs, like allergic rhinitis, acute and chronic rhinitis, cough and asthma. Modern pharmacological studies that have anti-tumor, anti-inflammation and anti-allergy activities. It is necessary to build up a method to determine its origin. We appraise the morphology (flowers, leaves, fruits and pollens) and histological anatomy for confirming its features. We set up a quantitative method of HPLC to determine the quantity of lupeol. This method has a high degree of sensitivity, reproducibility, precision and recovery rate. The result shows that the lupeol content of Centipeda minima is 0.00313~0.00424％. In setting up fingerprint, we use above mentioned HPLC method to observe the peak height ratio of the chosen 7 peaks and use different solvent to extract C. minima for observing the spectra of different extraction. These can help us to identify Centipeda species. The entire internal transcribed spacer (ITS) region between 18S and 26S ribosomal DNA of C. minima is amplified by polymerase chain reaction (PCR). The length of PCR product is 854 bp. The length of ITS1 is 255 bp and ITS2 is 222 bp. The G+C contents of ITS1 and ITS2 regions are 50.6 % and 51.8 %, respectively. ITS region is defferent in each species, even they are the same genus. So it is usually used to identify as differences to the plants. In this study, we try to find a best method to appraise C. minima and our aim is to find hepatoprotective effect for this plant.