| Summary: | 碩士 === 中國醫藥大學 === 醫學研究所碩士班 === 95 === Hepatic encephalopathy is a neuropsychiatric disorder and its pathogenesis is still not understood. The purpose of this study is designed to evaluate the time change of cerebral apoptosis by hyperammonemia in rats using the bile duct ligation model.
The male Wistar rats receiving bile duct ligation or not were studied in 1, 2, 3 weeks, respectively.
The terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick-end labeling (TUNEL) method was used to identify the formation of apoptotic cells. The apoptotic ratio was calculated and the distribution of apoptosis was inspected in the pyriform cortex, basal ganglia and hippocampus territory . The rats were divided into four groups including Group I : the control group with bile duct manipulation , Group II : bile duct ligation for one week . Group III two weeks after bile duct ligation. Group IV three weeks after bile duct ligation.
Results of this study, the proportion of apoptosis increased with the duration of bile duct ligation and reached a maximum after about two weeks . The mean values of the apoptotic ratio in hippocampus were zero in group I, 3.2 % in group II , 8.9 % in group III and 7.9 % in groupIV.
The distribution of apoptosis differed in the pyriform cortex, basal ganglia and hippocampus according to the duration of time of the hyperammonemia..
In the bile duct ligation model of rats, apoptosis observed in the brain is increased the time of hyperammonemia, reaching the peak at 2 weeks. The maximum formation of apoptosis is distributed at hippocampus than other parts of brain.
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