| Summary: | 碩士 === 中華大學 === 機械與航太工程研究所 === 95 === Nano/micro-structured or patterned surface opens new capabilities for biological sensing, cell manipulation and tissue engineering. This study aims to fabricate nano/micro-structured surface on PS materials by hot embossing followed by laser ablation method, and to investigate the effects of nano/micro-structured surface on the adhesion and growth of mouse myoblast cell line (C2C12). The optimum hot embossing condition for PS materials is the pressing temperature at 130℃ and 45℃ for lower and upper mold respectively, under a load of 700 Kg with press duration of 60 sec.. The sticking problem between the mold and PS materials can be significantly improved under the optimum conditions. Hot embossing at a lower temperature (< 125℃ for lower mold or < 40℃ for upper mold ) or the applied load less than 600 Kg leads to severe structure defects on the surface. The hot embossing carried out at a higher temperature (>135℃ for lower mold or > 50℃ for upper mold) or with press duration over 120 seconds results in distortion of the samples and bubbles formation in PS materials. The micro-scale holes or grooves with a diameter of 25, 50 and 100 μm were produced on nano-grooved PS surface by ArF laser ablation process. C2C12 cells were found to be well aligned along the nano-grooved PS surface. As the arrayed holes or grooves with a diameter larger than 50μm, the cells still allow adhering and growing inside these structures. However, the cells can be effectively isolated or prohibited from adhering and growing into these structures when the arrayed holes or grooves with a diameter of 25μm. This indicated that the nano-patterned combined with micro-structured surface provides sufficient flexibility to manipulate cell adhesion and growth behavior. Key words: nano/micro-structured surface, cell adhesion, hot embossing
|
|---|
