Hyaluronic acid production by flocculated/adhered immobilized streptococcus zooepidemicus

• Main Authors: Shiue Heh Sheng, 薛鶴聲
• Other Authors: 陳志平
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/72943519197418402157

碩士 === 長庚大學 === 生化與生醫工程研究所 === 95 === 英文摘要 The purpose of this research is to immobilize Streptococcus zooepidemicus by adsorption onto three different support, i.e Loofa sponge, polyurethane and poly ethylene terephthalate, for production of hyaluronic acid. The optimum flocculation concentration of chitosan for cells was 0.125%, and the cell flocculation percentage nder this situation was around 80%.From results of repeated batch fermentation, poly ethylene terephthalate demonstrates the best operation stability in each repeated batch cycle and was chosen for further bioreactor study. The best immobilization condition with 15 g/l initial biomass loading, and 10 ml/min circulation velocity. The cell immobilization efficiency approached 100% in this case. The best batch fermentation condition is 37 ℃, pH 7.0, 300 rpm, and 10 g/l initial glucose concentration. This system can produce 0.929 g/l hyaluronic acid and the productivity and glucose yield are 0.106 g/h/l and 0.093 g/g, respectively. Repeated batch fermentation experiments showed that immobilized cells could be reused for up to nine times and hyaluronic acid concentration can be maintained at 1.0 g/l. Contimuous culture fermentation experiments showed that HA concentration.