The SNOs in BRCA2/BRCA1 and their genetic susceptibility of oral cancer in Taiwan

• Main Authors: Ke Wen, Shao, 邵可雯
• Other Authors: 鄭恩加
• Format: Others
• Language: en_US
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/77143103369380250969

碩士 === 長庚大學 === 醫學生物技術研究所 === 95 === In Taiwan, the incidence of oral cancer has become the 6th leading cancer, the 4th leading cancer in male (2006), and is still increasing in the recent years. The association of carcinogen exposure (alcohol, betel quid, cigarette smoking) with oral cancer has been widely reported. However, the patients who have no expose to those carcinogens also exist. It implies that genomic background may also affect the incidence of oral cancer. Many evidences reveal that the mutations or certain SNPs in BRCA1 or BRCA2, which play important roles in DNA repair mechanism, affect breast cancer formation. In this study, we investigate whether the SNPs of these two genes also associate with the susceptibility of oral cancer. Total of 140 oral cancer patients and 88 normal individuals were recruited for study. The blood DNA were extracted and subjected to genotype analysis of 30 alleles in BRCA1 and BRCA2 by using TaqMan 5’-nuclease assay method. Results show that only rs1148321 (p=0.01) and rs542551 (p=0.02) in BRCA2 were statistically differential distributed between normal and cancer groups. In rs1148321, the A/T or T/T genotype in the cancer group showed higher frequency, with the odds ratio 2.09 (95% confidence interval: 1.18-3.68). In rs542551, the A/G or G/G genotype in the cancer group showed higher frequency, with the odds ratio 1.95 (95% confidence interval: 1.10-3.44). These SNPs have no association with multiple cancer development, neither clinico- patholgical phenotype of oral cancer. These results suggest that SNPs of rs1148321 and rs542551 in BRCA2 may be contributing factors for the early development of oral cancer, however, independent with the later steps of the cancer progression.