| Summary: | 碩士 === 長庚大學 === 基礎醫學研究所 === 95 === Previously, over expression of nucleophosmin (NPM)/B23 can increase DNA repair activity caused by UV treatment and inhibit apoptosis. But knockdown B23 expression can raise apoptosis. Use technology of siRNA, I produce stable transfected clone in MGH-U1 cells, first our observe basal function; include doubling time, saturation, density and invasion. And then observe sub-G1 by flow cytometry, when after UV irradiation, exhibit knockdown B23 can apparently raise apoptosis.
Previously, the hnRNP and B23 are abunt in nuclear matrix, so we want observe whether B23 of nuclar matrix can regulate expression of nuclear matrix proteins are altered and whether effect localization of nuclear matrix proteins.
GRP78 can regulate function of both AKT and NF-κB that can promote cell survival, inhibit apoptosis. And Both B23 and GRP78 whether have physiological significance or B23 can regulate function of GRP78 still need to prove.
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