Development of co-culture system for ethanol production from starch by Aspergillus awamori, Rhizopus japonicus, and Saccharomyces cerevisiae IR-2

• Main Authors: sky chen, 陳彥勳
• Other Authors: Liu yu-ko
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/88966592440635898633

碩士 === 長庚大學 === 化工與材料工程研究所 === 95 === Environmental pollution and agricultural wastes have spread all over the world in recent years so we, in order to do effective use of the offal. The purpose for study potapo starch to regard as substrate material and then utilile Aspergillus awamori and Rhizopus Japonicus of fungers to co-culture.It will make starch to come into being glucose after saccharifiction.Afterward, we use efficiency better saccharomycete- Saccharomyces cerevisiae IR-2 to research .Add S. cerevisiae IR-2 into the fungous co-suspension system in the best saccharification time (most reduce sugar), and let the system to become three strains of microorganisms co-suspension system.To find the suitable condition for culture the three strains of microorganisms system to go on different proportion and the amount of spores to addtion. At the same time,the system can avoid co-suspension during the saccharification process that accumulate the large amount reduce sugar make the damage of feedback inhibition,the saccharification and ferment processes can go on at the same moment. In the study,the best proportion to add A. awamor and R. japonicus is 7.5: 2.5,the large amount reduce sugar in the 28th hour and we get 2.152 mg/ml reduce sugar ,so we will go on next experiment that we find out the best addtion amount is 109 spores/ml to receive the concentration of best addtion by the experimental result. Follow-up the experiment , in which the best concentration for addtion is 0.1 x108 cell/ml on 12hr hour. It can get 0.57% of ethanol concentration. Transfer rotational speed for 100 rpm on 36th hour can up to 1.25%