Biochemical Characterization of C9orf75 and H3P
碩士 === 國立中正大學 === 分子生物研究所 === 95 === Abstract C9orf75 and H3P were identified as a PP1-binding protein by yeast two-hybridization system. Both proteins contain one PP1-binding motif. C9orf75 consists of 136 amino acids, while H3P contains 186 amino acids. Although C9orf75 and H3P have been proved as...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2009
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| Online Access: | http://ndltd.ncl.edu.tw/handle/61515034366842774095 |
| Summary: | 碩士 === 國立中正大學 === 分子生物研究所 === 95 === Abstract
C9orf75 and H3P were identified as a PP1-binding protein by yeast two-hybridization system. Both proteins contain one PP1-binding motif. C9orf75 consists of 136 amino acids, while H3P contains 186 amino acids. Although C9orf75 and H3P have been proved as the PP1-binding protein by GST pull-down assay and co-immunoprecipitation, it remained unknown whether both can modulate the catalytic activity of PP1. In this study, I characterized C9orf75 and H3P activities toward inhibition of PP1 activity. The results indicated that C9orf75 is a weaker inhibitor of PP1 and H3P cannot inhibit the PP1 activity, but enhances its activity.
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