| Summary: | 碩士 === 國立中正大學 === 分子生物研究所 === 95 === Human polyomavirus, JC virus (JCV), is a neurotropic DNA virus. Infection of JCV may lead to a fatal central nervous system (CNS) dymyelinating disease-- Progressive Multifocal Leukoencephalopathy (PML), common in AIDS patients or immunosuppressed individuals. The transcriptional control region (TCR) of JCV is highly variable which contains promoter and enhancer. In addition to virus classification, TCR may play an important role in determination of tissue tropism and viral life cycle. In the past few years, our laboratory have isolated ten genotypes with different TCRs of JCV Taiwan subtypes. The specific aim of this thesis was to study the influence of different TCRs in viral replication. By using PCR and gene cloning to replace TCR of TW3 with different TCRs, full length viral genome of different JCV Taiwan subtypes were obtained. Virions were produced after transfecting viral genome into cells. In addition, quantitative PCR was employed to determine virus copy number. Analysis of influences of viral infection of prototype (Mad-1), archetype (CY) and Taiwan various subtypes of JC virus was accomplished Western blot and immunofluorescence assay after infection of SVG-A cells with equal amount of virus. The results found that subtype TW5 and TW11 showed stronger activities of viral replication than other subtypes.
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