| Summary: | 碩士 === 元智大學 === 化學工程與材料科學學系 === 94 === This work used PAN and PES ultrafiltration membranes both MWCO, 100 kDa to separate proteins from binary solutions. Two reagents, bovine serum albumin (BSA) and brovine haemoglobin (HB) were separated via the ultrafiltration process. The study was divided into two parts. First, we used the Design-expert experiment software for analyzing single protein (BSA) filtration data, then we used dead-end and cross-flow modules to separate binary proteins and added adsorbent to improve the separation ability via selective adsorption. Finally, we used blocking filtration law to analyze fouling mechanism.
The design-expert experiment analysis show an increase in the single protein rejection and flux decline at pH < pI. Experiments of dead-end ultrafiltration showed that there is high separation factor at 10 psi and pH 7.10. It was shown that salt will results in a low flux decline and high separation factor. Cross-flow ultrafiltration experiments revealed a 20% less flux decline than the dead-end module, and the critical flux appeared at a pressure of 10 to 20 psi. The separation factor at pH 7.50 and 7.10 was the best. In the case of, the concentration ratio being 5 : 1 ( BSA : HB ) and pH ³ 7.10, the initial filtration mechanism is caused by internal blocking and later is responsible for cake fouling. When pH < 7.10, the blocking mechanism was complex and a model was used to find out the fouling time and fouling flux. The use of montmorillonite to selevtivly adsorb HB can increase the separation factor of HB and BSA.
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