Recovery and Separation of Bioproducts from Treated Fermentation Liquors by Solvent Extraction

• Main Authors: Huei-Li Chen, 陳惠莉
• Other Authors: 莊瑞鑫
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/72262310793199463789

碩士 === 元智大學 === 化學工程與材料科學學系 === 94 === In this paper, the extractive separation and recovery of bioagents from fermentation broth was studied. Batch equilibrium experiments were first conducted to find the optimal conditions for extractive separation. By performing liquid-liquid extraction with an organic solvent, we found that the best conditions were obtained when the bioagent concentration was 2000 mg/L in the aqueous phase at pH 8 and ethyl acetate was used as the solvent. The forward extraction was completed in 18 h, with 99% extraction of bioagents, but only 21% bioagent was extracted using n-hexane. On the other hand, the experimental results indicated that a better extraction efficiency could be obtained from treated fermentation broth when Aliquat 336 dissolved in n-hexane was used as the extractant. By using two organic solvents (ethyl acetate and n-hexane) in a microporous membrane-based stirred cell, a slow extraction of bioagents from the aqueous phase was observed even at low concentration. The extraction by the solvent ethyl acetate and n-hexane was 40% and 15%, respeatively. The extraction efficiency of n-hexane could not increase because of the small membrane area. In the microporous hollow fiber membrane device, the dominant effect was the adsorption. The adsorption capacity of hydrophobic membrane for bioagent was 3.9 g/m2 membrane. Ethanol was used for the desorption of the bioagent resulting in its purification. Bioagents isolated by solvent extraction was characterized by mass and NMR spectroscopy. The results showed that bioagents would bind with quaternary ammonium salt, leading to no emulsion formation.