Bio-activated Modified Poly (ε- caprolactone) (PCL): (I) Enhancing cell growth on PCL surface with nanometer roughness (II) Effects of cell migration on Hyaluronic acid/chitosan/PCL-based film surface

• Main Authors: Chien-Hung Hsieh, 謝健宏
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/95869111160875925317

碩士 === 國立雲林科技大學 === 工業化學與災害防治研究所 === 94 === Poly (ε- caprolactone) (PCL) films are improved their bioactivity for tissue engineering by grafting chitosan (CS) and Hyaluronic acid (HA). The surface properties of the extracellular matrix play vital roles in cellular behavior such as adhesion, spreading, proliferation and migration. While cell attachment and migration to a desired areas are mainly mediated by surface molecular interaction, cell morphology and orientation are significantly affected by the topographical cues of the substrate. The results suggest that high degree nano-roughness nano-CS/PCL films highly promote the growth and proliferation of fibroblasts, HGF and HDF, in-vitro. Graftting hyaluronic acid with chitosan could obviously promote the cell migration. These study also indicated that ordered and micropatterned on substrates can pattern cell, and guide cell alignment and oriented guides fibroblast migration to a desired areas. The new biomaterials, nano-CS/PCL, HA/CS/PCL, are developed by grafting a bioactive polymer onto biodegradable polymers. Biodegradable polymer scaffolds can be modified using this new technology as a new method for engineering soft tissues.