| Summary: | 碩士 === 國立陽明大學 === 藥理學研究所 === 94 === The principal aim of this study was to determine whether the polyphenol resveratrol can act as a neuroprotectant against a retinal ischemic insult. Such an insult is thought to play an important role in glaucomatous opticneuropathy and diabetic retinopathy, and so on.
It has been previously reported that matrix metalloproteinases (MMP)-9 is related to retinal ischemia. Although MMPs are essential for physiological extracellular matrix (ECM) remodeling, excessive levels of MMPs have been reported to degrade ECM and cause cell death including retinal ganglion cells indexed by Thy-1 mRNA. A polyphenol resveratrol can protect brain from ischemia damage by its antioxidative activity. In a tumor cell line study, it has been shown that resveratrol can decrease activity and level of MMP-9.
Present results have indicated that ischemia plus reperfusion caused retinal damage characterized by a decline in electroretinogram (ERG) b-wave amplitude, alteration in the localization of MMP-9 immunohistochemistry. A previous report also indicated that reduction of Thy-1 mRNA was associated, as well as an upregulation of MMP-9 mRNA. What is more, these damage were blunted when resveratrol (0.01 mM, but not 0.1 mM) was applied 24 hours before ischemia.
This study supports a neuroprotective role for resveratrol, acting through above mentioned mechanisms.
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