Characterization and Generation of Antibodies to Human Shen-Dan and Fibulin 4

• Main Authors: Cheng-Ta Han, 韓政達
• Other Authors: Fen-Hwa Wong
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/65480952810367648858

碩士 === 國立陽明大學 === 遺傳學研究所 === 94 === Abstract From the real-time PCR data it was shown that mRNA of human SD and its ligands HEDJ and fibulin 4, were over-expressed in the tissues of esophageal cancer patients. The mRNA of human SD and fibulin 4 also can be detected in esophageal cancer cell line. It suggests SD and its ligands HEDJ and fibulin 4 might have functions in esophageal cancer. In this project, we want to verify the expression of human SD and fibulin 4 proteins in esophageal cancer. We cloned human SD from TE-1 esophageal cancer cell line, and screen for antibodies can recognize human SD from 10 SD monoclonal antibodies that have already generated by Dr. Fann’s Lab. We found five antibodies could against human SD, and use three of them to detect human SD expression in six esophageal cancer cell lines. We also used immunoprecipitation and western blotting assay and immunohistochemistry staining to detect human SD protein expression in esophageal cancer cells. We could not detect human SD protein expression. To study fibulin 4 protein expression in esophageal cancer cell lines, we raised the antisera from rabbits, and the antisera could recognize fibulin 4 fusion protein.