| Summary: | 碩士 === 國立陽明大學 === 熱帶醫學研究所 === 94 === Molecular studies have demonstrated that c-Jun N-terminal kinase (JNK) is related to inflammation, embryonic development, cell proliferation, oncogenic transformation and apoptosis. JNK is a member of mitogen activated protein kinase (MAPK) family. Therefore to investigate if the JNK is involved in immune signaling is an important issue to look insight the immune regulation in mosquitoes. In this study, primers based on the sequence extracted from the Anopheles gambiae database were used to clone an Aedes aegypti JNK (AaJNK) cDNA fragment. An AaJNK cDNA fragment was isolated with Rapid Amplified cDNA End-PCR (RACE-PCR). Based on the Northern Blot analysis, the full-length of AaJNK mRNA is around 3,500 bps, but only 1,497 bps of AaJNK cDNA containing an open reading fram have been cloned and sequenced. Twelve protein kinase domains and a phosphorlation site (T-Y-P) were identified in the sequence. The expression of AaJNK was enhanced from 6 hours on after bacterial challenge. This result indicated that AaJNK was related to immunity in mosquitoes. Nevertheless, the productions of Aedes aegypti NO synthase (AaNOS) and antibacterial peptides (defensin and cecropin) are not regulated by AaJNK examined with dsRNAi of AaJNK and JNK specific inhibitor SP600125. This is the first identification of mosquito JNK involved in bacterial induction. However, its target genes responding to stimulation is required a further investigation.
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