CHARACTERIZATION OF NEOISLETS GENERATED FROM HUMAN PANCREATIC DUCTAL PROGENITOR/STEM CELLS

• Main Authors: Chia-Hsiang Chen, 陳家祥
• Other Authors: Tien-Hua Chen
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/50844467329425491961

碩士 === 國立陽明大學 === 解剖暨細胞生物學研究所 === 94 === The potential for the use of stem/progenitor cells for the restoration of injured or diseased tissues has garnered much interest recently, establishing a new field of research called regenerative medicine. Here we reported the isolation, propagation, and characterization of a population of ductal stem cells isolated from adult human donor pancreases. The aims of the present study were to improvise a methodology for obtaining large numbers of islets in vitro and to characterize their status for islet cell transplantation. The identity of neoislets was confirmed by immunofluorescent labeling and analysis of the gene expression for endocrine markers by RT-PCR. The islet population obtained was analyzed by image analysis and insulin secretion in response to glucose. The present study describes an improved method for obtaining a constant supply of large numbers of islet from pancreatic ductal stem cell cultures. The newly generated islets undergo functional maturation indicating their suitability for transplantation.