| Summary: | 碩士 === 國立陽明大學 === 解剖暨細胞生物學研究所 === 94 === Myocardial infarction is one of the leading cause of public mortality. Nowadays, stem cell transplantation shows great promise as a treatment of dysfunctional heart failure. Mesenchymal stem cells (MSCs) derived from human bone marrow underwent cardiomyogenic differentiation after 5-azacytidine exposures in vitro; however, treatment with 5-azacytidine remains question because of its cytotoxicity. In addition, mouse embryonic stem cells treated with transforming growth factor β2 (TGF-β2) exhibited spontaneous beating and expressed cardiac-specific proteins. In this study, we have found that after exposure to TGF-β2, MSCs obtained from Wharton’s jelly of human umbilical cord have the capacity to differentiate into cardiomyocytes. After exposure to TGF-β2, the expression of myogenic transcription factors, myogenin and MyoD1, was increased at the first 6 days. The expression of C-troponin I and connexin 43 was also increased in the first 6 days. These results suggested that TGF-β2 may play a role in the early stage of cardiomyogenic and myogenic differentiation. It is possible that other factors may be involved in this process. Additional factors may be necessary to fully commit these cells to cardiomyocytes.
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