Hyaluronan- oligosaccharide induces the expression of membrane- type 1 matrix metalloproteinase and CD44 cleavage in breast cancer cell invasion

• Main Authors: Jheng-Yi Chen, 陳政義
• Other Authors: Hwai-Shi Wang
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/08199631146164461791

碩士 === 國立陽明大學 === 解剖暨細胞生物學研究所 === 94 === Matrix metalloproteases (MMPs) are a group of proteases that are involved in extracellular matrix degradation. Since MMPs can degrade extracellular matrix, they can participate in tumor cell invasion and migration. Contemporary research divides the MMP family into two categories: soluble-type MMPs and membrane-type MMPs (MT-MMP). MT1-MMP is often expressed in invasive cancer cells and in endothelial cells during angiogenesis. CD44, a transmembrane receptor for hyaluronic acid (HA), is implicated in various adhesion-dependent cellular processes including cell migration, tumor cell metastasis and invasion. Recent studies have shown that CD44 expressed in cancer cells can be proteolytically cleaved at the ectodomain by MMPs family, This process of inducing CD44 de-adhesion is through metalloproteinase interaction and that CD44 cleavage plays a critical role in cancer cell migration. In this report, we have identified a new function of HA, namely its involvement in the induction of MT1-MMP expression in MDA-MB-435S breast cancer cells and induce CD44 cleavage to increase cell mobility and mediation of cellular migration during the invasion process. Based on these results we propose that one function of HA in tumor cells can induce MT1-MMP expression, which, at least for some tumor cell types, may be a critical step in the formation of metastatic colonies. Key words：Hyaluronan、CD44、CD44ICD、MT1-MMP、 breast cancer cells、cell migration