| Summary: | 碩士 === 國立陽明大學 === 生醫光電工程研究所 === 94 === EHEC (Enterohaemorrhagic E. coli) and Shigella belong to the families of Enterobacteriaceae. Ingestion of few EHEC and Shigella can cause diarrhea and shigellosis. Both bacteria bring patients inflammatory and severe bloody diarrhea, even death. EHEC causes actin pedestals of the host cells beneath the bacteria after infection of gut epithelial cells. But EHEC does not enter host cells after infection. And Shigella infects, invades, multiplies and moves in intestinal epithelial cells. Owing to Shigella has no flagella, Shigella modifies host molecular mechanisms to form actin tail behind the bacteria in order to have movement ability. But until now, there is no reference indicating the correlation between actin tail formation and membrane infection process. Here we use gene gun to bombard two different bacteria, EHEC and Shigella, into cells to avoid entry through normal infection process. According to our results, bombarded EHEC does not from pedestal in cells; and bombarded Shigella does not replicate or form actin tail in host cells. Thus we can speculate that Shigella must interact with cell surface and then can replicate and induce actin tail formation for movement.
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