| Summary: | 碩士 === 國立陽明大學 === 生物資訊研究所 === 94 === High-throughput experiments combined with network analysis could systematically construct the response model of organism behavior. We proposed to use high-throughput microarray analysis to study the heat shock response in Halobacterium sp. NRC-1 from the mRNA level. Also, the microarray expression data were integrated with NRC-1 interaction and KEGG metabolism networks via the public Cytoscape tool and KEGGtoCyto plugin developed in this study to systematically analyze the biomodules in NRC-1 heat shock response. In this study, we detected 5 of the 14 predicted heat shock protein genes, cctA, cctB, hsp1, hsp5 and dpsA were up-regulated upon temperature upshift. Hsp5 had a 7 folds increase which was the first rank up-regulated gene among all genes. But protease lon was down-regulated in the 15 minutes heat shock treatment. In the translation module, most of the ribosomal protein genes showed the trend of down-regulation. In the transcription module, RNA polymerase subunit E’ gene, rpoE’, showed the trend of up-regulation. Of the 7 general transcription factor Ⅱ B (TFB) genes, the expression of tfbB and tfbG were increased. According to an observation of metabolism network, some of the critical pyruvate related aerobic energy production enzymes were down-regulated in heat shock. The expression of most ATP synthase subunits were decreased. At the same time, the arginine fermentation energy production pathway related genes, arcABC, showed the trend of up-regulation. Taken together, the results suggested that NRC-1 may generate energy via the arginine deiminase pathway instead of respiration energy production pathway in heat shock condition. In this study, we found that NRC-1 heat shock response is complicated. Most of the results need further experimental validation. We expect to integrate more high-throughput heat shock data to construct and refine a more exquisite heat shock response.
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