Molecular cloning and characterization of a novel miz1-targeting protein

• Main Authors: Jeng-Kuan Jwo, 卓政寬
• Other Authors: Jim C. Fong
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/03452901747682500224

碩士 === 國立陽明大學 === 生化暨分子生物研究所 === 94 === Her-2/neu is one of the most important oncogenes in breast cancer. Her-2/neu is overexpressed in 30% of invasive breast cancers and is associated with an aggressive tumor phenotype, poor prognosis, shorter time for disease progression and shortened overall survival. Expression of high levels of Her-2/neu is sufficient to induce transformation of human mammary epithelial cells. Moreover, Her-2/neu overexpression enhances the invasive and metastatic phenotype of breast cancer cells and promotes resistance to chemo- and endocrine-therapies. Previous studies showed that novel gene H41 was found to be overexpressed in Her-2/neu-overexpressing human breast cancer MCF-7, but the biological function of H41 remained unknown. The present study indicates that H41-overexpressing MCF-7 turns out to be more invasive but H41 overexpression has no effect on the growth curve of MCF-7. Moreover, H41 overexpression inhibits cobalt- but anoxia-induced glucose uptake in MCF-7 stable clones. Inhibition of cobalt-induced glucose transport by H41 overexpression is mainly due to inhibition of GLUT1 expression in response to cobalt treatment. These findings indicate that H41 may play a role in the process of Her-2/neu-induced cell progression and regulates glucose uptake in human breast cancer.